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The adjusting collimator for the Fluorescent holographic microscope
Hlaváčová, Kateřina ; Jákl, Petr (referee) ; Dostál, Zbyněk (advisor)
For the proper function of the Fluorescence olographic microscope, it is necessary to adjust all the optical components of the microscope. Furthermore, the precise adjustment is the very critical condition for proper imaging of the Coherence-controlled holographic microscope. Therefore, it is necessary to create a sight collimator for these microscopes for their adjustment. The fluorescence holographic microscope is based on an interference and holographic principles, whose history is mentioned in the theoretical part of the thesis. The existing state of the art of laser sight collimators and their use in practice is also mentioned. The optical and mechanical design of the laser sight collimator and its realization are described in the next part of the thesis. The software for detecting the black sight cross was created for the use of the laser sight collimator in practice. The software is necessary to evaluate the correctness of the alignment of the adjusted microscope. The descriptions of the adjustment procedures for the laser sight collimator and for the Fluorescence holographic microscope are mentioned in the last part of the thesis. These procedures are necessary for proper manipulation and use with the proposed laser sight collimator.
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Study of thin film organic materials thickness
Hegerová, Lucie ; Veselý, Michal (referee) ; Zmeškal, Oldřich (advisor)
The diploma thesis deals with the determination of thickness and refractive index of thin organic films using image analysis. In the theoretical part there are described principles of the methods, which are used to prepare the films (spin coating, inkjet printing, vapour deposition), the characteristics of thin films, ways of finding out the thickness and refractive index of substances (weight methods, electric methods, method based on measurement of absorption coefficient of light, interference microscopy, ellipsometry) and also image analysis (harmonic and wavelet analysis). Interference microscope Epival - Interpako (Carl Zeiss Jena), digital camera Nikon Coolpix 5400 and computer were used for the determination of thickness and refractive index. The thicknesses of layers were set on the basis of interference images of edges and grooves – both from the side of the metal contact and the side of underlying glass. The refractive indices of thin layers were then set using the recorded figures. In the final part of the thesis there are discussed the results of interference images photographed along the full length of the aluminium contact which are used for measuring electrical characteristics of DPP structures. The produces are thicknesses and refractive indices of individual layers.
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Study of refractive index dispersion dependences with using of interference microscopy
Schmiedová, Veronika ; Veselý, Michal (referee) ; Zmeškal, Oldřich (advisor)
The master´s thesis deals with the study of optical properties of thin transparent layers on the organic materials (PPV, P3HT, TiO2, DPP) and especially with the determination of dispersion dependences of refractive index of prepared thin layers. In the theoretical part there are described principles of deposition thin layers of the analyzed materials and their properties. In addition, there are also described methods of optical properties measurements (optical and interference microscopy and ellipsometry). The combination of interference microscope with digital camera was used for determination of refractive index. The image analysis was used for the determination of parameters (with help of the software HarFA). The images of thin layers surfaces were analyzed from the side of the metal contact as well as from the side of glass. In conclusion, there are presented results of the refractive index of the thin layers obtained from the measured values.
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Design of the imaging part of the coherence-controlled holographic microscope
Dvořák, Vladislav ; Dostál, Zbyněk (referee) ; Špaček, Matěj (advisor)
The Experimental Biophotonics Research group at Brno University of Technology has been involved in the development of Coherence controlled holographic microscope (CCHM). For the purposes of further development in this field, it is necessary to build an open laboratory set-up of the microscope, which has only been available in a commercial design. The objective of this thesis is to design and assemble its imaging unit. The first part of the thesis is dedicated to a general overview of microscopic methods, with an emphasis on interference microscopy and principle of CCHM microscope. In the second part, different optical set-up concepts are discussed and compared. Then, the optical components are selected and the unknown parameters of these components are experimentally measured. With the use of measured parameters, the optical tracing and mechanical design are introduced. The imaging unit has been assembled based on the suggested design and experimentally verified.
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The adjusting collimator for the Fluorescent holographic microscope
Hlaváčová, Kateřina ; Jákl, Petr (referee) ; Dostál, Zbyněk (advisor)
For the proper function of the Fluorescence olographic microscope, it is necessary to adjust all the optical components of the microscope. Furthermore, the precise adjustment is the very critical condition for proper imaging of the Coherence-controlled holographic microscope. Therefore, it is necessary to create a sight collimator for these microscopes for their adjustment. The fluorescence holographic microscope is based on an interference and holographic principles, whose history is mentioned in the theoretical part of the thesis. The existing state of the art of laser sight collimators and their use in practice is also mentioned. The optical and mechanical design of the laser sight collimator and its realization are described in the next part of the thesis. The software for detecting the black sight cross was created for the use of the laser sight collimator in practice. The software is necessary to evaluate the correctness of the alignment of the adjusted microscope. The descriptions of the adjustment procedures for the laser sight collimator and for the Fluorescence holographic microscope are mentioned in the last part of the thesis. These procedures are necessary for proper manipulation and use with the proposed laser sight collimator.
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Study of refractive index dispersion dependences with using of interference microscopy
Schmiedová, Veronika ; Veselý, Michal (referee) ; Zmeškal, Oldřich (advisor)
The master´s thesis deals with the study of optical properties of thin transparent layers on the organic materials (PPV, P3HT, TiO2, DPP) and especially with the determination of dispersion dependences of refractive index of prepared thin layers. In the theoretical part there are described principles of deposition thin layers of the analyzed materials and their properties. In addition, there are also described methods of optical properties measurements (optical and interference microscopy and ellipsometry). The combination of interference microscope with digital camera was used for determination of refractive index. The image analysis was used for the determination of parameters (with help of the software HarFA). The images of thin layers surfaces were analyzed from the side of the metal contact as well as from the side of glass. In conclusion, there are presented results of the refractive index of the thin layers obtained from the measured values.
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Study of thin film organic materials thickness
Hegerová, Lucie ; Veselý, Michal (referee) ; Zmeškal, Oldřich (advisor)
The diploma thesis deals with the determination of thickness and refractive index of thin organic films using image analysis. In the theoretical part there are described principles of the methods, which are used to prepare the films (spin coating, inkjet printing, vapour deposition), the characteristics of thin films, ways of finding out the thickness and refractive index of substances (weight methods, electric methods, method based on measurement of absorption coefficient of light, interference microscopy, ellipsometry) and also image analysis (harmonic and wavelet analysis). Interference microscope Epival - Interpako (Carl Zeiss Jena), digital camera Nikon Coolpix 5400 and computer were used for the determination of thickness and refractive index. The thicknesses of layers were set on the basis of interference images of edges and grooves – both from the side of the metal contact and the side of underlying glass. The refractive indices of thin layers were then set using the recorded figures. In the final part of the thesis there are discussed the results of interference images photographed along the full length of the aluminium contact which are used for measuring electrical characteristics of DPP structures. The produces are thicknesses and refractive indices of individual layers.
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Study of thin films refractive index by interference microscopy
Procházka, Petr ; Veselý, Michal (referee) ; Zmeškal, Oldřich (advisor)
This bachelor thesis is focused to measure of spectral dependences of refractive index in thin layers. The object of this thesis was measurement of dispersion dependences of refractive index. The theoretical part deals with present methods of refractive index measurements and quantities associated with. These methods were ordered from the easiest to the most complex methods. Intention of own work was reached results by the very easy method of interference mi-croscopy. Thin layers of various organic matters were investigated by optical microscope and main-ly by interference microscope to determine their optical attributes – refractive index and his dis-persion dependences. There were images, which were made by interference microscope and digital camera. These images were subjected to image analysis by computer program HarFA. Dispersion dependences of refractive index were proved by elaboration by this computer pro-gram.
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